Volume 1, 2020

Neutrophil Response to Cyclophosphamide Predicts Resilience to Age-related Learning Impairment

Marianne Bjorner, Lida Zhu, Warren Ladiges

Abstract: Ability to respond to stress, defined as resilience, was measured by white blood cell counts in various age C57BL/6 mice receiving a nonlethal dose of cyclophosphamide (CYP). Neutrophil counts dipped and then rebounded in a consistent and age-dependent manner. Low neutrophil rebound correlated with improved learning in middle age mice suggesting CYP-induced neutrophil response may predict resilience to aging.

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Short Term Sleep Deprivation Predicts Pharmcological Resilience Response to Aging

Juan Wang, Clarice Forbes, Neva Hahn, Warren Ladiges

Abstract: The ability of an organism to respond to physical stress with increasing age, defined as physical resilience, was measured by the effect of short term sleep deprivation (SD) in 17 month old CB6F1 male mice. Sleep loss induces histone deacetylation (HDAC), resulting in cognitive dysfunction.  These mice were treated with phenylbuterate (PBA), an HDAC inhibitor, and then put through two physiological assessments. SD resistant mice had a weaker grip strength, but higher running distances than SD sensitive mice, suggesting these two physiological assessments have different pathway connections with PBA targeted hippocampal learning.

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GHK Peptide Prevents Sleep Deprived Learning Impairment in Mice

Katie Nickel, Warren Ladiges, Lida Zhu

Abstract: Sleep deprivation is known to cause memory impairment and is associated with inflammation and cell damage linked to neurodegenerative diseases. GHK (glycyl-L-histidyl-L-lysine) is a naturally occurring tripeptide found in mammalian plasma. GHK has anti-inflammatory activity and can pass through the blood brain barrier suggesting the potential to prevent neuroinflammation associated with sleep deprivation. In this study, mice were injected with 15mg/kg GHK per day for five days and sleep deprived on the last two days of treatment. Sleep deprived mice treated with GHK did not show the acute learning impairment seen in sleep deprived mice treated with saline. GHK prevented an increase in MCP-1 and nitric oxide levels in the hippocampus of sleep deprived mice suggesting that anti-inflammatory and anti-reactive nitrogen species activity could be a therapeutic target for learning impairment associated with short term sleep deprivation.

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